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16s rrna gene-pyrosequencing (v1-v3)  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc 16s rrna gene-pyrosequencing (v1-v3)
    Summary of studies examining the effects of probiotic co-supplementation to H. pylori eradication on the human gut microbiota.
    16s Rrna Gene Pyrosequencing (V1 V3), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/16s+rrna+gene-pyrosequencing+(v1-v3)/16s+rrna+pyrosequencing/pmc09373750-0-45-47
    Average 90 stars, based on 1 article reviews
    16s rrna gene-pyrosequencing (v1-v3) - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "The double-edged sword of probiotic supplementation on gut microbiota structure in Helicobacter pylori management"

    Article Title: The double-edged sword of probiotic supplementation on gut microbiota structure in Helicobacter pylori management

    Journal: Gut Microbes

    doi: 10.1080/19490976.2022.2108655

    Summary of studies examining the effects of probiotic co-supplementation to H. pylori eradication on the human gut microbiota.
    Figure Legend Snippet: Summary of studies examining the effects of probiotic co-supplementation to H. pylori eradication on the human gut microbiota.

    Techniques Used: Sequencing, Capsules, Maltodextrin

    Related Articles

    Biomarker Discovery:

    Article Title: Integrative Neuroimmune Role of the Parasympathetic Nervous System, Vagus Nerve and Gut Microbiota in Stress Modulation: A Narrative Review
    Article Snippet: .. 4 , Human case–control study comparing fecal microbiota in 72 Parkinson’s disease patients and 72 healthy controls , 16S rRNA gene pyrosequencing (V1–V3 regions); statistical analysis using generalized linear models. No treatment or dosing applied , Parkinson’s disease patients had a 77.6% reduction in Prevotellaceae abundance. Decreased Prevotellaceae was associated with PD diagnosis, while Enterobacteriaceae abundance correlated with postural instability and gait difficulty , Altered gut microbiota may influence disease through interactions with the enteric nervous system and vagus nerve, both early targets of α-synuclein pathology in Parkinson’s disease , [ ] . .. 5 , Mice with hepatocellular carcinoma; liver vagotomy, CD8+ T cell manipulation, microbiota transfer , Hepatic vagotomy; pharmacologic vagal activation; CD8+ T cell depletion; Chrm3 knockout; microbiota transplantation from HCC donors , Vagotomy → ↓ liver tumor growth, ↓ fatigue, ↓ anxiety. Vagal stimulation → ↑ tumor progression via immune suppression. Microbiota from HCC donors → impaired behavior and immunity , Vagal acetylcholine → CHRM3 receptor on CD8+ T cells → ↓ anti-tumor immunity. Gut microbiota + vagus nerve → regulate liver immune response and behavior , [ ] .

    Bacteria:

    Article Title: From Chihuahua to Saint-Bernard: how did digestion and microbiota evolve with dog sizes
    Article Snippet: .. Helicobacter spp are presents and could be pathogens Duodenum et Jejunum: Predominance of Eubacterium, Bacteroides, Clostridium, Fusobacterium, Bifidobacterium and Lactobacillus spp Ileum: Bacteroides, Eubacterium, Fusobacterium, Clostridium, Lactobacillus and Bifidobacterium predominate among the anaerobe bacteria There is also aerobe and facultative anaerobe bacteria: Streptococcus, Staphylococcus, Pasteurella, Escherichia and Enterobacter High density and diversity, Predominant bacteria are from Bacteroides, Bifidobacterium, Fusobacterium, Peptostreptococcus, Eubacterium, Clostridium, Peptococcus and Lactobacillus N/A Handl et al., 2011 12 N/A 0.7- 10.2 10 breeds N/A N/A N/A N/A 16S rRNA gene pyrosequencing (V1-V3 region) N/A N/A N/A Actinobacteria: 1.8% Bacteroidetes: 2.2% Firmicutes: 95% Fusobacteria: 0.3% Suchodolski, 2011 N/A N/A N/A N/A N/A N/A N/A N/A Review (or unpublished data) N/A 16S rRNA gene analysis: 2 archaeal phyla: Crenarchaeota et Euryarchaeota. .. Most abundant class: Methanobacteria (Methanosphaera et Methanobrevibacter) N/A 454 pyrosequencing: 4 fungal phyla with 90% Ascomycota > 5% Neocallimastigomycota Metagenomic sequencing: Archaea represents 1.1% of total microbiota (methanogens are the most abundant) Viruses are mainly bacteriophages and dsDNA viruses represent 0.4% of detected sequences Jia et al., 2010 8 N/A 4-13 N/A Commercial diet 18.2 9.9 CF: 1.6 SF: 5.8 IF: 1.2 FISH DGGE N/A N/A N/A Firmicutes: 31% Actinobacteria: 12% Bacteroidetes: <1% (108 cells/g feces) References N= Body weight (kg) Age (years) Breed Food Methods Gut microbiota composition Type of food Protein (%) Fat (%) Fiber (%) Stomach Small intestine Large intestine Stools Suchodolski et al., 2009 N/A N/A N/A N/A N/A N/A N/A 16S rRNA pyrosequencing N/A Jejunum mucosa: Proteobacteria: 46.7% Firmicutes: 15% Actinobacteria: 11.2% Spirochaetes: 14.2% Bacteroidetes: 6.2% Fusobacteria: 5.4% Tenericutes, Cyanobacteria, Verrucomicrobia and Chloroflexi: < 0.1% Gammaproteobacteria: 40% Spirochaetes: 13% Actinobacteria: 12% Clostridia: 10% Bacteroides: 6% Fusobacteria: 4% Alphaproteobacteria: 3% N/A N/A Biagi, Cipollini, & Zaghini, 2008 8 N/A N/A N/A N/A N/A N/A N/A Plate count N/A N/A N/A (Log CFU/mL) Lactobacilli: 7.9 Coliforms: 7.7 Enterococci: 7.5 Suchodolski et al., 2008b 64 N/A N/A N/A N/A N/A N/A N/A Illumina 16S rRNA gene sequencing N/A Fungi DNA detected in 60.9% of dogs and significatively increased in mucosal samples (82.8%) compared to luminal samples (42.9%): mainly Ascomycota and Basidiomycota.

    Medications:

    Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature
    Article Snippet: .. Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported. .. Stadlbauer 2017 [ ] , Austria , N = 30 n = 15 PD group GFR (ml/min): 7.9 (7.3; 14.0); Dialysis vintage: 25 (15-74) months. Age: 62yrs (54-69), 80% male. n = 15 HD group GFR (ml/min): 6.0 (5.9; 9.3); Dialysis vintage: 70 (40-197) months, Kt/V not reported. Age: 61yrs (54-71), 67% male; Ethnicity not reported. , N = 21 GFR (ml/min): 77.6 (73.4; 86.6). Age: 58 yrs (53-62), 43% male. Ethnicity not reported. , Self-sampled; Stored at -80°C , Not reported , Participants from HD and PD groups treated with phosphate binders, PPI, immunosuppressive drugs. Small number of controls ( n =2) also treated with PPI. , SILVA , 16S ribosomal RNA gene sequencing (V1-V2 region) Illumina MiSeq , ↓ α-diversity in PD and HD group compared to controls (Chao1 index, p <0.05, similar results reported for observed species/OTUs and phylogenic diversity) β-diversity significantly different between HD vs. controls p =0.012 and PD vs. controls p =0.003 (ANOSIM test based on Bray Curtis, weighted and unweighted UniFrac metrics) , Not reported.

    Sequencing:

    Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature
    Article Snippet: .. Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported. .. Stadlbauer 2017 [ ] , Austria , N = 30 n = 15 PD group GFR (ml/min): 7.9 (7.3; 14.0); Dialysis vintage: 25 (15-74) months. Age: 62yrs (54-69), 80% male. n = 15 HD group GFR (ml/min): 6.0 (5.9; 9.3); Dialysis vintage: 70 (40-197) months, Kt/V not reported. Age: 61yrs (54-71), 67% male; Ethnicity not reported. , N = 21 GFR (ml/min): 77.6 (73.4; 86.6). Age: 58 yrs (53-62), 43% male. Ethnicity not reported. , Self-sampled; Stored at -80°C , Not reported , Participants from HD and PD groups treated with phosphate binders, PPI, immunosuppressive drugs. Small number of controls ( n =2) also treated with PPI. , SILVA , 16S ribosomal RNA gene sequencing (V1-V2 region) Illumina MiSeq , ↓ α-diversity in PD and HD group compared to controls (Chao1 index, p <0.05, similar results reported for observed species/OTUs and phylogenic diversity) β-diversity significantly different between HD vs. controls p =0.012 and PD vs. controls p =0.003 (ANOSIM test based on Bray Curtis, weighted and unweighted UniFrac metrics) , Not reported.

    Article Title: High-Throughput Molecular Characterization of the Microbiome in Breast Implant-Associated Anaplastic Large Cell Lymphoma and Peri-Implant Benign Seromas.
    Article Snippet: .. Both Walker et al. and Hu et al. employed 16S rRNA gene sequencing (V1–V3 regions) with the same taxonomic classifier but different sequencing methods (Illumina NGS vs. FLX pyrosequencing) [27,28]. ..

    Article Title: High-Throughput Molecular Characterization of the Microbiome in Breast Implant-Associated Anaplastic Large Cell Lymphoma and Peri-Implant Benign Seromas
    Article Snippet: .. Both Walker et al. and Hu et al. employed 16S rRNA gene sequencing (V1–V3 regions) with the same taxonomic classifier but different sequencing methods (Illumina NGS vs. FLX pyrosequencing) [ , ]. ..

    Next-Generation Sequencing:

    Article Title: High-Throughput Molecular Characterization of the Microbiome in Breast Implant-Associated Anaplastic Large Cell Lymphoma and Peri-Implant Benign Seromas.
    Article Snippet: .. Both Walker et al. and Hu et al. employed 16S rRNA gene sequencing (V1–V3 regions) with the same taxonomic classifier but different sequencing methods (Illumina NGS vs. FLX pyrosequencing) [27,28]. ..

    Article Title: High-Throughput Molecular Characterization of the Microbiome in Breast Implant-Associated Anaplastic Large Cell Lymphoma and Peri-Implant Benign Seromas
    Article Snippet: .. Both Walker et al. and Hu et al. employed 16S rRNA gene sequencing (V1–V3 regions) with the same taxonomic classifier but different sequencing methods (Illumina NGS vs. FLX pyrosequencing) [ , ]. ..

    other:

    Article Title: The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome
    Article Snippet: Panulirus ornatus (Spiny lobster) / Arthropoda , Sea water , 16S rRNA gene amplicon (V1-V3) pyrosequencing , Ooi et al., 2019.

    Article Title: The double-edged sword of probiotic supplementation on gut microbiota structure in Helicobacter pylori management
    Article Snippet: Oh et al., 2016 , 10 subjects in each group , Amoxicillin 1 g bid, clarithromycin 500 mg bid, lansoprazole 30 mg bid, 14 days , Streptococcus faecium 9 × 10 8 , Bacillus subtilis 1 × 10 8 , twice daily, 14 days , 16S rRNA gene-pyrosequencing (V1-V3) , 2 weeks , Citrobacter, Klebsiella, Pseudomonas , and Escherichia , NS.



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    16s rrna gene-pyrosequencing (v1-v3)  (Pyrosequencing Inc)
    90
    Pyrosequencing Inc 16s rrna gene-pyrosequencing (v1-v3)
    Summary of studies examining the effects of probiotic co-supplementation to H. pylori eradication on the human gut microbiota.
    16s Rrna Gene Pyrosequencing (V1 V3), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/16s+rrna+gene-pyrosequencing+(v1-v3)/16s+rrna+pyrosequencing/pmc09373750-0-45-47
    Average 90 stars, based on 1 article reviews
    16s rrna gene-pyrosequencing (v1-v3) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    Summary of studies examining the effects of probiotic co-supplementation to H. pylori eradication on the human gut microbiota.

    Journal: Gut Microbes

    Article Title: The double-edged sword of probiotic supplementation on gut microbiota structure in Helicobacter pylori management

    doi: 10.1080/19490976.2022.2108655

    Figure Lengend Snippet: Summary of studies examining the effects of probiotic co-supplementation to H. pylori eradication on the human gut microbiota.

    Article Snippet: Oh et al., 2016 , 10 subjects in each group , Amoxicillin 1 g bid, clarithromycin 500 mg bid, lansoprazole 30 mg bid, 14 days , Streptococcus faecium 9 × 10 8 , Bacillus subtilis 1 × 10 8 , twice daily, 14 days , 16S rRNA gene-pyrosequencing (V1-V3) , 2 weeks , Citrobacter, Klebsiella, Pseudomonas , and Escherichia , NS.

    Techniques: Sequencing, Capsules, Maltodextrin